IntelliQube® Product Specifications
Users can expect minimal well-to-well variability in Array Tape, providing The IntelliQube demonstrated a total standard deviation (SD) of 0.087 cycles when 768 replicate wells each containing 2048 copies of a NIST calibrated human – gDNA reference standard were analyzed for a non- transcribed locus in hgDNA.
The IntelliQube can support assays across a large range of input concentrations while maintaining data integrity. When analyzing a 1000 bp synthetic target serially diluted across eight orders of magnitude, the IntelliQube demonstrated a linear dynamic range of quantifications between 4x107 to 40 copies/reaction.
Minimal differences in target quality can be accurately resolved between reaction wells on the IntelliQube. Using NIST calibrated hgDNA reference standard, reactions were prepared to represent 1.2 fold differences in template concentration. When analyzed for a non-transcribed locus in hgDNA, the IntelliQube demonstrated the ability to resolve 1.2 fold differences with greater than 97% sensitivity and specificity.
The IntelliQube provides the sensitivity necessary to amplify and detect even 1-2 copies of target DNA in a reaction well. Although the theoretical limit of detection (LOD) provided by Poisson statistics is limited to three molecules, IntelliQube has demonstrated the ability to detect as little as 1-2 molecules of hgDNA per reaction.
IntelliQube Performance Specifications
24,960 data points
For Research Use Only. Not for use in diagnostic procedures.