Microbial Detection and Quantification

Nucleic Acid Testing (NAT) is a powerful tool for detecting and quantifying microorganisms. NAT is used in many fields, ranging from food safety to human diagnostics. The quantitative detection of pathogens can assist in the quality control of industrial processes to ensure there is no microbial contamination present. Development of accurate and economical methods for microbial quantitative analysis is therefore of upmost importance for ensuring the safety of the food we eat and the proper diagnosis of infections.

The IntelliQube® System is designed to address this need by producing accurate and reliable results with walk-away automation that substantially reduces reagent expenditures and labor requirements. In a recent study, three commercially available microbial quantitation kits were successfully miniaturized from 20 µL to 1.6 µL reaction volumes.

Microbial Figure 1

Figure 1: Comparison of the standard curves generated on the IntelliQube and ViiA™ 7 for the Escherichia coli (All Strains), All pathogenic Salmonella Species, and Human Papillomavirus 16 kits. The concentration range for these curves is 105 to 10 control template copies per reaction. The standard curve is represented by the blue line with uncertainty indicated by the dashed red lines above and below the standard curve. The plots represent eight replicates and four replicates per dilution for the InteliQube and ViiA 7, respectively. With the exception of E.coli tested on the ViiA 7, all standard curves passed the CLSI linearity test. All plots were generated using GenEx PCR Analysis Software.

The results show comparable results with other leading instrumentation on the market, while significantly reducing reagent use.

Primerdesign Standard Kit (Assay Only)
$384.20
$0.307
$3.842
PerfeCTa qPCR ToughMix Low Rox Quanta BioSciences
$77.50
$0.062
$0.775
Total
$461.70
$0.369
$4.617

Table 1: Reagent cost comparison between 1.6 µL reactions in Array Tape® and 20 µL reactions in 384-well PCR plates.

Click here to download the complete study!