DNAble® Isothermal DNA Amplification and Detection Technology From Douglas Scientific
DNAble Isothermal DNA Amplification Chemistry
The DNAble isothermal DNA amplification and detection technology represents a new paradigm in real time nucleic acid analysis and has the potential to impact the world of molecular biology.
This new DNAble assay solution developed by EnviroLogix™ delivers results equivalent to quantitative polymerase chain reaction (qPCR) in less than 10 minutes. These attributes make this solution equally valuable for point-of-use testing as well as extremely high throughput applications.
Rapid Isothermal Nucleic Acid Amplification Technology
DNAble is a very rapid, highly specific isothermal nucleic acid amplification technology that can amplify both RNA and DNA targets with single base resolution a billion-fold in 5-10 minutes at a single and constant temperature. The proprietary chemistry produces highly specific, rapid, multiplexed, quantitative results rivaling qPCR. By combining the DNAble chemistry with Douglas Scientific’s Array Tape® Platform, laboratories can save up to 80-90% on reagents while increasing throughput to hundreds of thousands of data points in a day.
Isothermal DNA Amplification Process
DNAble’s unique amplification process begins with a nicking enzyme that nicks the DNA structure at specific recognition sites. A polymerase then extends from the “nicked” site displacing the original opened single strand. These single strands hybridize with both the forward and reverse DNAble templates and the reaction begins to occur at an exponential rate yielding a detectable target in less than 10 minutes.
Additional information on the Douglas Scientific/EnviroLogix Collaborative Agreement to revolutionize qualitative and quantitative nucleic acid analysis solutions is available in the linked announcement.
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