IntelliQube® Product Specifications

IntelliQube
Figure 1. Cq Uniformity <0.09 SD
IntelliQube
Figure 2. Linear dynamic range of quantification from 4x107 to 40 copies/reaction
IntelliQube
Figure 3. 1.2 fold resolution

Cq Uniformity

Users can expect minimal well-to-well variability in Array Tape, providing The IntelliQube demonstrated a total standard deviation (SD) of 0.087 cycles when 768 replicate wells each containing 2048 copies of a NIST calibrated human – gDNA reference standard were analyzed for a non- transcribed locus in hgDNA.

Dynamic Range

The IntelliQube can support assays across a large range of input concentrations while maintaining data integrity. When analyzing a 1000 bp synthetic target serially diluted across eight orders of magnitude, the IntelliQube demonstrated a linear dynamic range of quantifications between 4x107 to 40 copies/reaction.

Resolution

Minimal differences in target quality can be accurately resolved between reaction wells on the IntelliQube. Using NIST calibrated hgDNA reference standard, reactions were prepared to represent 1.2 fold differences in template concentration. When analyzed for a non-transcribed locus in hgDNA, the IntelliQube demonstrated the ability to resolve 1.2 fold differences with greater than 97% sensitivity and specificity.

Sensitivity

The IntelliQube provides the sensitivity necessary to amplify and detect even 1-2 copies of target DNA in a reaction well. Although the theoretical limit of detection (LOD) provided by Poisson statistics is limited to three molecules, IntelliQube has demonstrated the ability to detect as little as 1-2 molecules of hgDNA per reaction.

IntelliQube Performance Specifications

Dimensions (W x D x H)
49" x 34" x 75" (with cart)
Weight
855 pounds
Input Voltage
120/240 VAC, 15/7.5 A
Frequency
50-60 Hz
Operating Temperature
15-30°C
Operating Humidity
20-80% Relative Humidity, non-condensing
Air Input
80-100 psi, bursts up to 15 SCFM
Water Input
Reverse Osmosis or Deionized
Certifications
Conforms to the European Machinery Directive (2006/42/EC) and the EMC Directive (2004/108/EC). For Research Use Only. Not for use in Diagnostic Procedures.
Estimated End-point PCR Throughput Per 8 Hours (With Hydrocycler2)
Sixty Five 384-well arrays
24,960 data points

For Research Use Only. Not for use in diagnostic procedures.